br Anti BrdU APC br Anti Annexin V APC
Anti-BrdU-APC
Anti-Annexin V-APC
Bacterial and Virus Strains
Chemically competent One shot Stbl3
Biological Samples
Patient-derived pancreatic cancer
Moores Cancer Center,
N/A
xenografts and organoid lines
University Y-27632 California
San Diego
Chemicals, Peptides, and Recombinant Proteins
Gemcitabine
Sigma
Cat# G6423
Critical Commercial Assays
NEBNext Ultra II DNA library prep kit
NucleoBond Xtra Maxi DNA puriÞcation kit
KAPA HiFi HotStart ReadyMIX
KAPA Biosystems
Cat# KK2602
QIAGEN Blood and Cell Culture DNA Midi Kit
Chromium Single Cell 30 GEM library and
gel bead kit v2
RNeasy Micro Plus kit
QIAGEN
IlluminaÕs TruSeq Stranded mRNA Sample
Illumina
Prep Kit
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REAGENT or RESOURCE
SOURCE
IDENTIFIER
NEBNext Ultra II Directional RNA Library
New England Biolabs
Prep Kit for Illumina
Superscript III
Invitrogen
Deposited Data
https://www.ncbi.nlm.nih.gov/geo/
ChIP-seq data
Custom code for CRISPR screen analysis
https://github.com/ucsd-ccbb/
Primary Msi2+ and Msi2- KPf/fC RNA-seq data
crispr_network_analysis.
https://www.ncbi.nlm.nih.gov/geo/
Genome-wide CRISPR screen data
https://www.ncbi.nlm.nih.gov/geo/
shRorc versus shControl KPf/fC RNA-seq data
https://www.ncbi.nlm.nih.gov/geo/
shRorc versus shControl KPf/fC H3K27ac
https://www.ncbi.nlm.nih.gov/geo/
ChIP-seq data
sgRorc versus sgNT human PDAC FG RNA-seq
https://www.ncbi.nlm.nih.gov/geo/
Single-cell sequencing of KPR172HC tumors
https://www.ncbi.nlm.nih.gov/geo/
Single-cell sequencing of KPf/fC tumors
https://www.ncbi.nlm.nih.gov/geo/
Experimental Models: Cell Lines
American Type Culture Collection
Human: FG Cells
Gifted by Dr. Andrew Lowy,
This paper
Mouse: KPf/fC
This paper
Mouse: REM2-KPf/fC
This paper
Experimental Models: Organisms/Strains
The Jackson Laboratory
The Jackson Laboratory
The Jackson Laboratory
Rorctm1Litt/J
Mouse: REM2 (Msi2eGFP/+) reporter
Mouse: NOD/SCID; NOD.CB17-Prkdcscid/J
Dr. Tyler Jacks
The Jackson Laboratory
Mouse: NSG; NOD.Cg-PrkdcscidIL2rgtm1Wji/SzJ
The Jackson Laboratory
Oligonucleotides
shRNA Autosomes targeting sequences- see Table S7
This paper
All primer sequences, see Table S7
This paper
Recombinant DNA
mouse GeCKO CRISPRv2 knockout
pooled library
pRSV/REV
pMDLg/pRRE
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REAGENT or RESOURCE
SOURCE
IDENTIFIER
pHCMVG
pLV-hU6-mPGK-red
Biosettia
Software and Algorithms
Kallisto
https://pachterlab.github.io/kallisto/
DESeq2
https://bioconductor.org/packages/
release/bioc/html/DESeq2.html
Limma
http://bioconductor.org/packages/
release/bioc/html/limma.html
Gene Set Enrichment Analysis (GSEA)
http://software.broadinstitute.org/
gsea/index.jsp
Bioconductor GSVA
https://bioconductor.org/packages/
release/bioc/html/GSVA.html
Langmead and Salzberg, 2012
https://sourceforge.net/projects/bowtie-
https://sourceforge.net/projects/samtools/
Broad Institute
https://broadinstitute.github.io/picard/
Aaron Quinlan Lab
https://github.com/genomecuration/JAMg/
SICER-df algorithm version 1.1
https://home.gwu.edu/ wpeng/Software.htm
Tophat2
https://ccb.jhu.edu/software/tophat/index.shtml
Cufßinks, Cuffdiff
http://cole-trapnell-lab.github.io/cufßinks/manual/
ChippeakAnno
http://bioconductor.org/packages/release/